The present study was focused on isolation of biosurfactant producing bacteria from environmental samples contaminated soil and uncontaminated water and assessing the potential of these isolates. Isolation of biosurfactant producing bacteria from tannery. Microbial biosurfactants, produced by fungi, yeast, and bacteria, are. Morrillb and tahir husaina biosurfactant producing nitratereducing bacteria nrb in anaerobic reservoir environments are closely associated with souring h. Production and extraction of biosurfactant was carried out using bacillus, lactobacilli, the parameter optimization for the better growth of the strain was carried out. Isolation and screening of biosurfactant producing bacteria. Detection of biosurfactant activity was carried out for 95 isolates using a drop collapse assay jain et al. Bacterial dna was extracted, and amplified by pcr method. Hence, the aim of this study is to isolate and identify efficient biosurfactant producing. Mj9 is a highly effective biosurfactant producing bacterium, the production purified and characterized was similar in structure to the rhamnolipid biosurfactant by tlc, ftir, and 1 h nmr.
Isolation and identification of lactobacilli from cheese. Water samples were serially diluted from 101 to 106, 1 ml of 104 to 106 dilutions were used to inoculate plate count agar plates by spread plate method. Pdf isolation of thermotolerant, halotolerant, facultative. Youssef nh, duncan ke, nagle dp, savage kn, knapp rm, mcinerney mj. View the article pdf and any associated supplements and figures for a period of 48 hours. Isolation and characterization of potent biosurfactant. A study on biosurfactant production in lactobacillus and. Isolation of biosurfactant producing bacteria from garbage soil. In the present research investigation, indigenous bacteria from cq1 to cq were isolated from soil collected from changqing oil field of xian, china. Mar 25, 2021 biosurfactants, a wide group of compounds produced by different microorganisms, generally have less toxicity and are more biodegradable than synthetic surfactants. Bacterial quantification bq quantification was performed following carlucci. Culture broth was centrifuged at 6000 rpm for 15 min and cell free. Jun 30, 2016 isolation of biosurfactant producing bacteria a 5 g crude oil contaminated soil sample was suspended in 95 ml sterile phosphate buffer solution pbs in a 250 ml conical flask and placed on shaker at 200 rpm at 20c for 12 h. Measurements of surface tension, critical micelle dilution and emulsifying activity indicated that the biosurfactant is produced as a primary metabolite.
This paper describes the isolation of a potent biosurfactant producing bacteria for use in enhance oil recovery. A direct thinlayer chromatography technique was used for a rapid characterization of biosurfactant producing bacterial colonies schulz et al. Bacteria are the main group of biosurfactant producing microorganisms, although it is also produced by some yeast and filamentous fungi. Measurements of surface tension, critical micelle dilution and emulsifying activity indicated that the biosurfactant is produced as a. Characterization of bacteria isolation of bacteria from. Isolation of biosurfactantproducing marine bacteria and. Bacterial quantification bq quantification was performed following carlucci et al. Hemolysis was used as a criterion for the primary isolation of biosurfactant producing bacteria.
Pdf isolation of biosurfactant producing bacteria from oil reservoirs. Isolation and characterization of biosurfactant producing. The bacterial isolates from crude oil and produced water samples were partially identified as pseudomonas spp. Pelagia research library screening and isolation of biosurfactant.
Two dirt samples were collected from oil contaminated site of garage. Furthermore, they used agarplates coated with di fferent pahs and. These 37 strains, together with 49 nonhaemolytic ones chosen at random, were studied for surface activity. Pdf biosurfactant isolation, production, purification. Pdf isolation and selection of biosurfactantproducing. Isolation and characterization of biosurfactantproducing bacteria. Biosurfactants are surfaceactive substances synthesized by living cells and as compared to synthetic surfactants, they are more effective, selective, environmental friendly and stable. This cell free culture broth was used for further assays. Oct 01, 2020 in the paper, a biosurfactant producing bacteria paracoccus sp. The cellfree extract was used as the sample for the oilspre. Hence, the isolation of microbial strains capable of biosurfactant production using soluble substrates is of interest. Results from screening methods revealed that hemolytic and bath assays are not reliable methods to check biosurfactant production because hemolytic assay relies on lysis of blood cells, which can be caused by compounds produced.
Materials and methods isolation of biosurfactant producing bacteria biosurfactant producing bact eria were isolated from soils contaminated with palm oil from a palm oil. Isolation of nitratereducing bacteria from an offshore. Isolation of biosurfactant producing bacteria from oil reservoirs. Isolation of biosurfactant producing bacteria from an oil.
Isolation of biosurfactantproducing bacteria from the. Therefore, isolation and identification of biosurfactant producing and hydrocarbon degrading bacteria are pivotal for effective bioremediation of hydrocarbon contaminated surface waters. Biosurfactant production and hydrocarbon degradation activity. A number of studies have reported the potential of bacillus species as biosurfactant producers and they produce lipopeptide type of biosurfactant. Qualitative analysis of biosurfactant production was identified by performing assays like hemolysis on blood agar, penetration assay and ctab agar assay.
Only 37 of 492 strains of different origins had haemolytic activity. Isolation and characterization of biosurfactantsproducing. Nov 03, 2018 the endophytic strain identified as bacillus pumilus 2a produce biosurfactant that is able to act as plantgrowth promoting agent. Evaluation of screening methods for the isolation of biosurfactant. The 34 isolates associated with bacillus, streptomyces, microbacterium, micrococcus, rhodococcus, pseudomonas, arthrobacter and staphylococcus genera retrieved from the three oil wells were identified as biosurfactant producing bacteria using the oilspreading and the drop collapse methods.
Appl isolation and selection of biosurfactant producing bacteria. Haemolysis has been used as an initial selection criterion for the primary isolation of surfactant producing bacteria. Nov 01, 2020 in the present research, biosurfactant production was studied in eight halophilic bacteria isolated from khewra salt mines. Effects of different ph, salinity and temperature on biosurfactant production were also studied. Five microliters of the cell free culture broth was transferred to the oil. Isolated microorganisms were identified based on the biochemical characterization as. One sample is from shakti garage, ahmedabad and another. Isolation of biosurfactant producing bacteria from garbage. The 3 isolates from cheese, yoghurt and silage were effective against isolated li and could produce biosurfactants. Drop collapse test drops of the cell free supernatant are placed on an oil coated. They have the properties of reducing surface tension. Biosurfactant production and characterization of bacillus sp. A number of studies have reported the potential of bacillus species as biosurfactant producers and they produce lipopeptide type of biosurfactant nitschke and pastore, 2004.
Isolation of biosurfactant producing bacteria from potwar oil fields. Comparison of methods to detect biosurfactant production by diverse microorganisms. Pdf isolation of biosurfactant producing bacteria from. Materials and methods microbial culture isolation of biosurfactant producing bacteria was performed according to francy et al. Endophytic bacteria isolated from chelidonium majus l. Ruwaida kuwait institute of scientific research, p. Isolation, characterization and identification of bacterial biosurfactant.
The present article is aimed to determine common cultivable surfactant producers from soil and contaminated oil, its purification and significance. Biosurfactant producing bacteria can be found in contaminated environments, such as soils receiving pesticide applications constantly, or in pesticides treatment systems where microorganisms are adapted to biodegrading pesticides. Isolation and characterization of biosurfactantproducing. Isolation and selection of biosurfactantproducing bacteria. Isolation of biosurfactant producing marine bacteria 783 maneerat, s.
Isolation of biosurfactant producing bacteria braz. Agronomy free fulltext a pesticide biopurification. Biosurfactant producing bacteria, biosurfactant, emulsification, microbial enhanced oil recovery meor. Isolation of isolation of biosurfactant producing bacteria from oil reservoirs a tabatabaee 1, m mazaheri assadi 2, a a noohi 1,v a sajadian 3 1faculty of science, research and technology, islamic azad university, iran 2 biotechnology center of iranian research organization for science and technology, iran 3research institute of petroleum industry, tehran, iran. These molecules reduce surface tension both aqueous. Isolation and enrichment of biosurfactant producing bacteria. Hence, the aim of this study is to isolate and identify efficient biosurfactant producing and diesel oil. Screening concepts for the isolation of biosurfactant producing microorganisms 3 willumsen and karlson3 isolated biosurfactant producing bacteria from soil which was contaminated with polyaromatic hydrocarbons pahs. Pdf isolation of biosurfactant producing bacteria from garbage soil. Isolation of bacterial community of each water environment. One sample is from shakti garage, ahmedabad and another dirt sample from new vallabh vidhyanagar garage, anand. The extraction and emulsification potency of the surfactant was performed in order to use as bioemulsifier in food priya and usharani, 2009.
Isolation of biosurfactant producing bacteria from tannery effluents in sokoto metropolis, nigeria abdullahi adamu p 1 p, udem j. They used pahamended liquid minimal medium for enrichment culture. The biosurfactant was recovered from the cell free culture supernatant by cold. Apr 03, 2015 bacteria that produce biosurfactant from crude oil and produced water sources were isolated, and partially identified. Madhura warde published on 20160317 download full article with reference data and citations. Mj9, was isolated from marine environment, and the biosurfactants and biodegradation were studied. Isolation of biosurfactant producing bacteria from the caspian sea and determination of their biosurfactant activity safari a 1, akbarzadehkhayavi m 2, roayaeiardakani m 3, motamedi h 3 1 anatomical science research center, kashan univ ersity of medical sciences, kashan, i. P1 strain from heavily petroleum hydrocarboncontaminated soil at. Isolation and screening of biosurfactant producing.
Characterization of a biosurfactantproducing bacteria. Only five strains, all of them haemolytic, tested positive. Bacteria isolation mineralbase mediums of di potassium hydrogen phosphate, ammonium chloride, manganese sulfate and 1 ml of micronutrients with ml of distilled water were used to isolate the bacteria. For production of biosurfactant, microbes were inoculated in 50ml of mineral salt media msm 3. Enhanced production of biosurfactant from bacillus subtilis.
Bioremediation of crude oil using bacteria has emerged as a green cleanup approach in. Isolation and screening of biosurfactantproducing bacteria. Jun 01, 2016 then, to analyze and identify the biosurfactant producing bacteria were cultured on a mineral salt medium. Ibrahim p 3 p 1 pmaryam abacha women and children hospital sokoto p 2 pdepartment of microbiology, federal university of technology, minna p 3. Isolation of potent biosurfactant producing bacteria from. Isolation and characterization of biosurfactant produced by. Morrillb and tahir husaina biosurfactant producing nitratereducing bacteria nrb in anaerobic reservoir environments are closely. To study the effect of initial ph on biosurfactant production by bacillus velenzensis, the initial ph of the medium was adjusted to 5.
Isolation of potent biosurfactant producing bacteria from oil. In article 12 walter, christoph syldatk and rudolf hausmann, 2010. Isolation and characterization of biosurfactant produced. Enumeration of biosurfactant producing microorganisms from oil contaminated soil in and around bangalore india. For instance, pacwaplociniczak and coworkers were able to isolate pseudomonas sp. The biosurfactant producing ability of the microorganisms isolated from the soil samples was investigated by hemolytic assay, drop collapse test, emulsification index and methylene blue agar plate method. The optimal conditions for bacterial growth were found to be ph 7 and temperature 30oc for strain b1 and ph 8 and temperature 30oc for strain b2. Isolation and identification of biosurfactantproducing. Evaluation of screening methods for the isolation of. Two biosurfactantproducing pseudomonas aeruginosa strains kisr c1 and kisr b1 were isolated from kuwaiti oilcontaminated soil, which resulted from the. Isolation and screening of microorganisms from oil contaminated soil samples with biosurfactant producing ability was investigated.
Pdf isolation and selection of biosurfactantproducing bacteria. Alternative methodology for isolation of biosurfactant. Selective isolation of potentially phosphatemobilizing. Screening and isolation of biosurfactant producing bacteria from petroleum. Researchers worldwide have been doing a lot of studies on biosurfactant producing bacteria for bioremediation purposes. Isolation of biosurfactant producing bacteria from oil.
Therefore the present investigation was undertaken to isolate biosurfactant producing bacteria from soil and to check the efficiency of biosurfactant in oil degeradation, ii. Isolation and screening of biosurfactant producing bacteria from oil contaminated soils in iraq doi. The biosurfactant production was screened by oil spreading technique 2. Biosurfactant production and hydrocarbon degradation. The highest biosurfactant activity was indicated by the largest diameter of the clear zone and the best isolates were selected for further study to. Pdf biosurfactants are amphiphilic compounds produced extracellularly by microorganisms on cell surfaces, or excreted extracellularly. Use of different methods for detection of thermophilic biosurfactant producing bacteria from hydrocarboncontaminated and bioremediated soils. Isolation and characterization of grampositive biosurfactant. Screening concepts for the isolation of biosurfactant producing microorganisms. The isolated bacteria were studied for antagonistic effects on environment isolated li, bacteriocin and biosurfactant production.
Pdf biosurfactants or surfaceactive compounds are produced by microoaganisms. These properties provide promising perspectives for application of biosurfactants. Four promising biosurfactant producers cq1, cq2, cq4, and cq were selected through primary screening among. Subsequently, bacterial cell free culture medium was acidified with 6 m hydrochloric acid to ph 2 and left overnight at 4 c. Isolation and characterization of biosurfactantbiopolymer. Screening concepts for the isolation of biosurfactant. Biosurfactant, ecofriendly, microorganism, biodegradable.
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